Mechanically Sheared Axially Swept Light-Sheet Microscopy

Lin, J.; Mehra, D.; Marin, Z.; Wang, X.; Borges, H. M.; Shen, Q.; Galecki, S.; Haug, J.; Dean, K. M.

doi:10.1101/2024.04.10.588892

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bioRxiv · 10.1101/2024.04.10.588892

Mechanically Sheared Axially Swept Light-Sheet Microscopy

Lin, J.·Mehra, D.·Marin, Z.·Wang, X.·Borges, H. M.·Shen, Q.·Galecki, S.·Haug, J.·Dean, K. M.

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Abstract

We present a mechanically sheared image acquisition format for upright and open-top light-sheet microscopes that automatically places data in its proper spatial context. This approach, which reduces computational post-processing and eliminates unnecessary interpolation or duplication of the data, is demonstrated on an upright variant of Axially Swept Light-Sheet Microscopy (ASLM) that achieves a field of view, measuring 774 x 435 microns, that is 3.2-fold larger than previous models and a raw and isotropic resolution of [~]420 nm. Combined, we demonstrate the power of this approach by imaging sub-diffraction beads, cleared biological tissues, and expanded specimens.

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Lin, J., Mehra, D., Marin, Z., Wang, X., Borges, H. M., Shen, Q., Galecki, S., Haug, J., Dean, K. M.. 2024-04-13. Mechanically Sheared Axially Swept Light-Sheet Microscopy. https://doi.org/10.1101/2024.04.10.588892

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