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bioRxiv · 10.1101/2024.04.10.588854

PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data

Blanchard, A. M.·Taylor, A.·Warry, A.·Shephard, F.·Curwen, A.·Leigh, J. A.·Emes, R. D.·Egan, S. A.
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Abstract

MotivationCurrent methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilitate real time analysis. SummaryWe have created an accessible web-based browser tool for visualisation and downstream analysis of high-throughput TIS data. This includes multiple interactive and sortable tables to aid the user to identify genes of interest, enabling the user to gain a greater understanding of the genes contributing to fitness in their experimental work. PIMMS-Dash permits researchers, with any level of bioinformatics knowledge, to interrogate data sets and generate publication quality figures. AvailabilityPIMMS-Dash is freely available and is accessible online at https://pimms-dashboard-uon.azurewebsites.net

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Blanchard, A. M., Taylor, A., Warry, A., Shephard, F., Curwen, A., Leigh, J. A., Emes, R. D., Egan, S. A.. 2024-04-14. PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data. https://doi.org/10.1101/2024.04.10.588854

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