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bioRxiv · 10.1101/2024.04.08.588511

Pattern formation along signaling gradients driven by active droplet behaviour of cell groups

Abstract

Gradients of extracellular signals organise cells in tissues. Although there are several models for how gradients can pattern cell behaviour, it is not clear how cells react to gradients when the population is undergoing 3D morphogenesis, in which cell-cell and cell-signal interactions are continually changing. Dictyostelium cells follow gradients of their nutritional source to feed and maintain their undifferentiated state. Using light sheet imaging to simultaneously monitor signaling, single cell and population dynamics, we show that the cells migrate towards nutritional gradients in swarms. As swarms advance, they deposit clumps of cells at the rear, triggering differentiation. Clump deposition is explained by a physical model in which cell swarms behave as active droplets: cells proliferate within the swarm, with clump shedding occurring at a critical population size, at which cells at the rear no longer perceive the gradient and are not retained by the emergent surface tension of the swarm. The droplet model predicts vortex motion of the cells within the swarm emerging from the local transfer of propulsion forces, a prediction validated by 3D tracking of single cells. This active fluid behaviour reveals a developmental mechanism we term "musical chairs" decision-making, in which the decision to proliferate or differentiate is determined by the position of a cell within the group as it bifurcates.

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BibTeXRIS

Ford, H. Z., Celora, G. L., Westbrook, E. R., Dalwadi, M. P., Walker, B. J., Baumann, H., Weijer, C. J., Pearce, P., Chubb, J. R.. 2024-04-09. Pattern formation along signaling gradients driven by active droplet behaviour of cell groups. https://doi.org/10.1101/2024.04.08.588511

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