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bioRxiv · 10.1101/2024.04.07.588437

A step-emulsion based digital-RPA for Pathogenic bacteria detection

Jin, J.·Lu, L.·Chen, H.·Wang, Y.·Zheng, G.
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Abstract

Foodborne bacteria are major causes that affect human health. Development of new method that could achieve Rapid, sensitive and quantitative detection of pathogen is urgently needed. In this research, a step emulsion microfluidic,combined with droplet-based digital-RPA, was developed to detect Vibrio parahaemolyticus, a major seafood-borne pathogenic bacteria. Specific and rapid detection of Vibrio parahaemolyticus in 30 min has been achieved by this new device, with a detection limit of 10 CFU/L, about 10-times lower than classical tube-based RPA. This device was demonstrated to be a promising tool for detection of pathogenic bacteria.

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Jin, J., Lu, L., Chen, H., Wang, Y., Zheng, G.. 2024-04-07. A step-emulsion based digital-RPA for Pathogenic bacteria detection. https://doi.org/10.1101/2024.04.07.588437

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