ROS Mediated the Photodynamic Antibacterial of Vibrio vulnificus by a DNA-Targeted Molecular Rotor Probe

Abstract

Vibrio vulnificus, a highly pathogenic Gram-negative bacterium, is capable of inducing sepsis, necrotizing fasciitis, and skin and soft tissue infections through contact with wounds. Currently, the majority of V. vulnificus strains have developed resistance to multiple drugs, highlighting the critical necessity for the development of novel therapeutics capable of effectively targeting and eradicating this bacterium. In recent years, material molecules have emerged as promising antimicrobial agents. This study introduces a novel molecular fluorescent probe, BDTP, which demonstrates a wide-ranging antimicrobial effect against both Gram-negative and Gram-positive bacteria while exhibiting minimal toxicity to normal mammalian cells. Of particular significance is BDTPs ability to rapidly detect V. vulnificus, bind to bacterial DNA, and exhibit fluorescence monitoring behavior. Furthermore, BDTP displays enhanced photodynamic antibacterial activity when exposed to white light irradiation. Under a low dose of white light (15mW cm-2), the killing efficiency of V. vulnificus irradiated with 4uM for 10 minutes was more than 99.8%. Moreover, it could significantly inhibit and eliminate the biofilm formed by V. vulnificus. It can induce the production of reactive oxygen species (ROS) in V. vulnificus cells, leading to bacterial cell damage. More importantly, BDTP significantly promoted the healing of infected wounds in an animal model of V. vulnificus infection. Therefore, BDTP shows great promise as a potent antibacterial agent against V. vulnificus infection.