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bioRxiv · 10.1101/2024.04.03.587821

The kinesin motor Kif9 regulates centriolar satellite positioning and mitotic progression.

Vicente, J. J.·Wagenbach, M.·Decarreau, J.·Zelter, A.·MacCoss, M. J.·Davis, T.·Wordeman, L.
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Abstract

Centrosomes are the principal microtubule-organizing centers of the cell and play an essential role in mitotic spindle function. Centrosome biogenesis is achieved by strict control of protein acquisition and phosphorylation prior to mitosis. Defects in this process promote fragmentation of pericentriolar material culminating in multipolar spindles and chromosome missegregation. Centriolar satellites, membrane-less aggrupations of proteins involved in the trafficking of proteins toward and away from the centrosome, are thought to contribute to centrosome biogenesis. Here we show that the microtubule plus-end directed kinesin motor Kif9 localizes to centriolar satellites and regulates their pericentrosomal localization during interphase. Lack of Kif9 leads to aggregation of satellites closer to the centrosome and increased centrosomal protein degradation that disrupts centrosome maturation and results in chromosome congression and segregation defects during mitosis. Our data reveal roles for Kif9 and centriolar satellites in the regulation of cellular proteostasis and mitosis.

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Vicente, J. J., Wagenbach, M., Decarreau, J., Zelter, A., MacCoss, M. J., Davis, T., Wordeman, L.. 2024-04-03. The kinesin motor Kif9 regulates centriolar satellite positioning and mitotic progression.. https://doi.org/10.1101/2024.04.03.587821

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