Intra-species quantification reveals differences in activity and sleep levels in the yellow fever mosquito, Aedes aegypti

Ajayi, O.; Susanto, E. E.; Wang, L.; Kennedy, J.; Ledezma, A.; Harris, A.; Smith, E. S.; Chakraborty, S.; Wynne, N. E.; Sylla, M.; Akorli, J.; Otoo, S.; Rose, N. H.; Vinauger, C.; Benoit, J. B.

doi:10.1101/2024.03.16.585223

bioRxiv · 10.1101/2024.03.16.585223

Intra-species quantification reveals differences in activity and sleep levels in the yellow fever mosquito, Aedes aegypti

Ajayi, O.·Susanto, E. E.·Wang, L.·Kennedy, J.·Ledezma, A.·Harris, A.·Smith, E. S.·Chakraborty, S.·Wynne, N. E.·Sylla, M.·Akorli, J.·Otoo, S.·Rose, N. H.·Vinauger, C.·Benoit, J. B.

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Abstract

Aedes aegypti is an important mosquito vector of human disease with a wide distribution across the globe. Climatic conditions and ecological pressure drive differences in the biology of several populations of this mosquito, including blood-feeding behavior and vector competence. However, no study has compared activity and/or sleep among different populations/lineages of Ae. aegypti. Having recently established sleep-like states in three mosquito species with observable differences in timing and amount of sleep among species, we investigated differences in activity and sleep levels among 17 Ae. aegypti lines drawn from both its native range in Africa and its invasive range across the global tropics. Activity monitoring indicates that all the lines show consistent diurnal activity, but significant differences in activity level, sleep amount, number of sleep bouts, and bout duration were observed among the lines. Variations in specific activity and sleep parameters were explained by differences in host preference, ancestry, and human population density for the lineages collected in Africa. This study provides evidence that the diurnal sleep and activity profiles for Ae. aegypti are consistent, but there are significant population differences for Ae. aegypti sleep and activity levels and interactions with humans may significantly impact mosquito activity and sleep.

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Ajayi, O., Susanto, E. E., Wang, L., Kennedy, J., Ledezma, A., Harris, A., Smith, E. S., Chakraborty, S., Wynne, N. E., Sylla, M., Akorli, J., Otoo, S., Rose, N. H., Vinauger, C., Benoit, J. B.. 2024-03-19. Intra-species quantification reveals differences in activity and sleep levels in the yellow fever mosquito, Aedes aegypti. https://doi.org/10.1101/2024.03.16.585223

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