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bioRxiv · 10.1101/2024.03.13.584835

Escherichia coli grown in cost-effective conical tubes produces more plasmid DNA

Marquez, M.·Chen, Q.·Cachaco, S.·Sui, H.·Imamichi, T.
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Abstract

Before the COVID-19 pandemic, we grew plasmid-transformed Escherichia coli in Falcon round-bottom-polypropylene tubes (F-Round-PP) and isolated plasmid using a Miniprep kit. When obtaining sufficient quantities of F-Round-PP became problematic during the pandemic and the inflation, we grew them using any available tubes. Notably, we observed that plasmid yield from cells grown in a cost-effective Oxford conical-polypropylene tubes (O-Conical-PP) was higher than that from F-Round-PP. We assessed the impact using O-Conical-PP and other conical brand tubes. As a result, the plasmid yield from O-Conical-PP (the list price is nearly 1/3 of F-Round-PP) was 1.5-fold higher than other PP tubes (p<0.001). We propose that researchers may need to re-assess the effectiveness of their laboratory supplies to optimize the budget during this inflationary period.

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Marquez, M., Chen, Q., Cachaco, S., Sui, H., Imamichi, T.. 2024-03-14. Escherichia coli grown in cost-effective conical tubes produces more plasmid DNA. https://doi.org/10.1101/2024.03.13.584835

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