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bioRxiv · 10.1101/2024.03.08.584098

High-resolution analysis of the treated coeliac disease microbiome reveals increased inter-individual variability

Abstract

BackgroundCoeliac disease (CeD) is an immune-mediated disorder primarily affecting the small intestine, characterised by an inflammatory immune reaction to dietary gluten. CeD onset results from a multifaceted interplay of genetic and environmental factors. While recent data show that alterations in gut microbiome composition could play an important role, many current studies are constrained by small sample sizes and limited resolution. MethodsTo address these limitations, we analysed faecal gut microbiota from two Dutch cohorts, CeDNN (128 treated CeD patients (tCeD), 106 controls) and the Lifelines Dutch Microbiome Project (24 self-reported tCeD, 654 controls), using shotgun metagenomic sequencing. Self-reported IBS (570 cases, 1710 controls) and IBD (93 cases, 465 controls) were used as comparative conditions of the gastrointestinal tract. Interindividual variation within the case and control groups was calculated at whole microbiome and strain level. Finally, species-specific gene repertoires were analysed in tCeD patients and controls. ResultsWithin-individual microbiome diversity was decreased in patients with self-reported IBS and IBD but not in tCeD patients. Each condition displayed a unique microbial pattern and, in addition to confirming previously reported microbiome associations, we identify an increase in the levels of Clostridium sp. CAG:253, Roseburia hominis, and Eggerthella lenta, amongst others. We further show that the observed changes can partially be explained by gluten-free diet adherence. We also observe increased interindividual variation of gut microbiome composition among tCeD patients and a higher bacterial mutation frequency in tCeD that contributes to higher interindividual variation at strain level. In addition, the immotile European subspecies of Eubacterium rectale, which has a distinct carbohydrate metabolism potential, was nearly absent in tCeD patients. ConclusionOur study sheds light on the complex interplay between the gut microbiome and CeD, revealing increased interindividual variation and strain-level variation in tCeD patients. These findings expand our understanding of the microbiomes role in intestinal health and disease. HighlightsO_LIThis is the largest microbiome collection of coeliac disease (CeD) patients assembled to date, providing insights into gut microbiome composition down to strain level. C_LIO_LICompared to controls, treated CeD (tCeD) patients adhering to a gluten-free diet show novel gut microbiome associations. C_LIO_LItCeD patients also have a less uniform gut microbiome than controls. C_LIO_LIBacteria display higher mutation frequency in tCeD compared to controls. C_LIO_LIHallmarks of the European subspecies of Eubacterium rectale are nearly absent in tCeD patients, implying selection at strain level. C_LI

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BibTeXRIS

Slager, J., Simpson, H. L., Gacesa, R., Chen, L., Gelderloos, J., Maatman, A., Wijmenga, C., Zhernakova, A., Fu, J., Weersma, R., Gonera, G., Jonkers, I. H., Withoff, S.. 2024-03-11. High-resolution analysis of the treated coeliac disease microbiome reveals increased inter-individual variability. https://doi.org/10.1101/2024.03.08.584098

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