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bioRxiv · 10.1101/2024.03.06.583732

Unravelling the Interplay of Nitrogen Nutrition and the Botrytis cinerea pectin lyase BcPNL1 in Modulating Arabidopsis thaliana Susceptibility

Daviere, A.·Voxeur, A.·Jolivet, S.·Vernhettes, S.·Soulie, M.-C.·Fagard, M.
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Abstract

In this study, we investigated the intricate interplay between nitrogen nutrition, and the dynamics of pectin degradation during plant-pathogen interactions, using Arabidopsis thaliana and Botrytis cinerea as a model pathosystem. Our findings revealed a noteworthy impact of nitrogen availability on the pectin degrading activity of the B. cinerea pectin lyase 1 (PNL) for which the mutant strains presented a reduced pathogenicity restored by complementation. More precisely, infected nitrogen-sufficient (high N) plants exhibited an increased release of PNL-derived oligogalacturonides compared to infected nitrogen-deficient (low N) plants. This correlated with an elevated expression of jasmonic acid repressor genes in high N plants, rendering them more susceptible to B. cinerea. Using{Delta} Bcpnl1 deletion mutants, we demonstrated that the increased production of BcPNL1 -derived oligogalacturonides under high N conditions was responsible for the increased expression of jasmonic acid repressor genes, significantly contributing to the higher susceptibility of high N plantsto B. cinerea. In conclusion, we demonstrated that BcPNL1 is a major pathogenicity factor during B. cinerea interaction that is affected by the plants N nutrition conditions.

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Daviere, A., Voxeur, A., Jolivet, S., Vernhettes, S., Soulie, M.-C., Fagard, M.. 2024-03-09. Unravelling the Interplay of Nitrogen Nutrition and the Botrytis cinerea pectin lyase BcPNL1 in Modulating Arabidopsis thaliana Susceptibility. https://doi.org/10.1101/2024.03.06.583732

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