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bioRxiv · 10.1101/2024.03.04.583397

Reconciling the importance of minerals for propagation of antibiotic resistance genes in the environment

Hendiani, S.·Carbajo, C.·Hansen, M. F.·Agbaje, O.·Arellano, P.·Verma, T.·Mandic Mulec, I.·Burmoelle, M.·Sand, K. K.
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Abstract

The role of mineral surfaces in environmental processes, particularly their influence on DNA preservation, biofilm formation, and genetic transfer, has garnered attention due to its implications for the spread of antibiotic resistance genes (ARg). Despite the recognized significance of mineral-mediated DNA transfer, this mechanism remains poorly understood. Here we investigate the intricate interplay between soil minerals, bacteria, and DNA, to better understand the mechanisms driving ARg propagation in natural environments. We here study the uptake of mineral adsorbed DNA into the natural competent bacteria b. subtilis and further explore the influence of minerals on the viability and subsequent biofilm formation of both b. subtilis and A. baylyi. We further adsorbed DNA to mineral surfaces and allowed biofilm formation while monitoring the propagation of the ARg through out the biofilms. All the results are set in context of mineral surface properties such as surface charge, charge densities and surface area. Our results showed that the surface properties of the mineral surfaces are highly influencing the transformation efficiencies, viability and biofilm formation where in particular a high number of positive charged surface sites enhance biofilm formation and viability and inhibit transformation. The influence of the mineral surfaces diminishes as the biofilm develops and propagation of mineral adsorbed ARg are seen widely across the mineral surfaces. Our results have implication for mitigations strategies and reconcile mineral surfaces as hot spots for the propagation of antibiotic resistance-which indeed can be driven by transformation in the absence of bacteria carrying the traits. In principle all it takes is one successful transfer event from a mineral adsorbed ARg.

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Hendiani, S., Carbajo, C., Hansen, M. F., Agbaje, O., Arellano, P., Verma, T., Mandic Mulec, I., Burmoelle, M., Sand, K. K.. 2024-03-05. Reconciling the importance of minerals for propagation of antibiotic resistance genes in the environment. https://doi.org/10.1101/2024.03.04.583397

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