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bioRxiv · 10.1101/2024.02.29.581642

Variables affecting acquisition and maintenance of operant ethanol self-administration in male and female Long-Evans rats

Wheeler, S. R.·Pitock, J. R.·Ayala, A. P.·Hou, S.·Soto, N. M. A.·Glover, E. J.
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Abstract

AimsThe goal of the present study was to determine the effect of prior experience with ethanol drinking and changes in session duration on the acquisition and maintenance of operant ethanol self-administration. MethodsAdult male and female Long-Evans rats were trained to operantly self-administer ethanol. A subset of male rats underwent intermittent-access two-bottle choice drinking in the home cage prior to operant training. Controls were given access to two bottles of water. Once fully trained in 30-min operant sessions, session duration was reduced to 15 min for all male and female rats. Differences between 30- and 15-min sessions were also assessed in a separate group of male and female rats trained to self-administer sucrose. ResultsNo differences were observed in acquisition rates, the magnitude of responding for ethanol, or total ethanol consumed between male rats allowed to drink ethanol in the home cage and those that remained ethanol naive prior to operant training. A significant decrease in appetitive and consummatory behaviors was observed in males trained to lever press for either ethanol or sucrose. Females exhibited a similar decrease in operant performance for sucrose, but their behavior was largely unchanged in response to changes in session duration when ethanol was the reinforcer. ConclusionsThese data suggest that the use of prior home cage ethanol drinking as an initiation procedure offers little advantage over no initiation procedure at all. Moreover, reducing operant session duration from 30-min to 15-min has the potential to decrease, rather than increase, levels of ethanol intake. Short summaryIntermittent-access two-bottle choice ethanol drinking offers no advantage as an initiation procedure for operant ethanol self-administration over animals that are ethanol-naive prior to training. In addition, shortening the operant session duration does not increase overall intake or promote binge-like patterns of intake for either ethanol or sucrose reinforcer.

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Wheeler, S. R., Pitock, J. R., Ayala, A. P., Hou, S., Soto, N. M. A., Glover, E. J.. 2024-03-02. Variables affecting acquisition and maintenance of operant ethanol self-administration in male and female Long-Evans rats. https://doi.org/10.1101/2024.02.29.581642

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