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bioRxiv · 10.1101/2024.02.23.581433

SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis

Opzoomer, J. W.·O'Sullivan, R.·Sufi, J.·Madsen, R. R.·Qin, X.·Basiarz, E.·Tape, C. J.
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Abstract

We present SIGNAL-seq (Split-pool Indexing siG-Nalling AnaLysis by sequencing): a multiplexed splitpool combinatorial barcoding method that simultaneously measures RNA and post-translational modifications (PTMs) in fixed single cells from 3D models. SIGNAL-seq PTM measurements are equivalent to mass cytometry and RNA gene detection is analogous to split-pool barcoding scRNA-seq. By measuring both mRNA ligand-receptor pairs and PTMs in single cells, SIGNAL-seq can simultaneously uncover inter- and intra-cellular regulation of tumour microenvironment plasticity.

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Opzoomer, J. W., O'Sullivan, R., Sufi, J., Madsen, R. R., Qin, X., Basiarz, E., Tape, C. J.. 2024-02-27. SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis. https://doi.org/10.1101/2024.02.23.581433

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