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bioRxiv · 10.1101/2024.02.20.580683

DCAF16-Based Covalent Handle for the Rational Design of Monovalent Degraders

Abstract

Targeted protein degradation with monovalent molecular glue degraders is a powerful therapeutic modality for eliminating disease causing proteins. However, rational design of molecular glue degraders remains challenging. In this study, we sought to identify a transplantable and linker-less covalent handle that could be appended onto the exit vector of various protein-targeting ligands to induce the degradation of their respective targets. Using the BET family inhibitor JQ1 as a testbed, we synthesized and screened a series of covalent JQ1 analogs and identified a vinylsulfonyl piperazine handle that led to the potent and selective degradation of BRD4 in cells. Through chemoproteomic profiling, we identified DCAF16 as the E3 ligase responsible for BRD4 degradation--an E3 ligase substrate receptor that has been previously covalently targeted for molecular glue-based degradation of BRD4. Interestingly, we demonstrated that this covalent handle can be transplanted across a diverse array of protein-targeting ligands spanning many different protein classes to induce the degradation of CDK4, the androgen receptor, BTK, SMARCA2/4, and BCR-ABL/c-ABL. Our study reveals a DCAF16-based covalent degradative and linker-less chemical handle that can be attached to protein-targeting ligands to induce the degradation of several different classes of protein targets.

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BibTeXRIS

Lim, M., Do Cong, T., Orr, L. M., Toriki, E. S., Kile, A. C., Papatzimas, J. W., Lee, E., Nomura, D. K.. 2024-02-22. DCAF16-Based Covalent Handle for the Rational Design of Monovalent Degraders. https://doi.org/10.1101/2024.02.20.580683

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