Tissue Tension and Strain as Indicators of Suction-mediated Cutaneous DNA Transfection: A Parametric Study

Jhumur, N. C.; Lallow, E. O.; Nachtigal, C.; Yeo, D.; Kwon, I.; Park, Y. K.; Roberts, C. C.; Zahn, J. D.; Shreiber, D. I.; Shan, J. W.; Singer, J. P.; Maslow, J. N.; Lin, H.

doi:10.1101/2024.02.15.580412

bioRxiv · 10.1101/2024.02.15.580412

Tissue Tension and Strain as Indicators of Suction-mediated Cutaneous DNA Transfection: A Parametric Study

Jhumur, N. C.·Lallow, E. O.·Nachtigal, C.·Yeo, D.·Kwon, I.·Park, Y. K.·Roberts, C. C.·Zahn, J. D.·Shreiber, D. I.·Shan, J. W.·Singer, J. P.·Maslow, J. N.·Lin, H.

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Abstract

Cutaneous suction-based transfection is a recently developed technique that is painless and simple-to-use for the delivery of DNA for nucleic-acid-based vaccines. The technique promises high efficiency for both antigen expression and immunogenicity as demonstrated in both animal studies and human clinical trials. To realize this promise, a parametric study and systematic evaluation on the efficacy of cutaneous suction as a transfection method was performed. Using Green Fluorescent Protein (GFP) plasmid expression as a transfection reporter in a rat model, the expression level as a function of both suction nozzle size and suction pressure was quantified. A numerical model was employed to compute skin deformation in terms of strain, which was used to correlate with GFP expression. Based on these results, two quantities, total integrated strain and tissue tension, are proposed as indicators of expression level that can be used to guide protocol development and optimization. These indicators are also discussed in relation to possible cellular uptake mechanisms.

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Jhumur, N. C., Lallow, E. O., Nachtigal, C., Yeo, D., Kwon, I., Park, Y. K., Roberts, C. C., Zahn, J. D., Shreiber, D. I., Shan, J. W., Singer, J. P., Maslow, J. N., Lin, H.. 2024-02-16. Tissue Tension and Strain as Indicators of Suction-mediated Cutaneous DNA Transfection: A Parametric Study. https://doi.org/10.1101/2024.02.15.580412

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