bioRxiv Science⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2024.02.14.580272

Molecular characterization and culture optimization of marine copepod Oithona dissimilis (Landberg, 1940) from Nagore coastal waters, Southern India

PILIYAN, R. P.·Perumal, S.·Barathan, B. P.·Meril, D.·Ragothaman, P.·Shanmugam, G.·Pachippan, P.
View original source ↗Export metadata ↓

Abstract

This study focused on identifying the cyclopoid copepod Oithona dissimilis found in the Nagore coastal waters of Southern India. Based on both morphological and molecular approaches to identify this species. Morphological characters were confirmed by examining the arrangement of setae and spines on the exopod of swimming legs 1-4. Molecular studies were performed using the CO 1 gene, which proved an effective marker for species identification. After identifying the Oithona dissimilis, reared them under laboratory conditions to determine the effects of various environmental parameters on their survival, nauplii production, and population density. Tested different temperatures, light intensity, pH, salinity, and diets and found that the optimum conditions for rearing Oithona dissimilis under laboratory conditions were a salinity of 25 PSU, a temperature range of 24-28{degrees} C, pH of 8, light intensity of 500 lux, and a mixed algal diet at a concentration of 30,000 cells/ml. The present study confirms the importance of accurate taxonomic identification for the Oithona group at the species level. Additionally, our findings show that rearing cyclopoid copepods under laboratory conditions that mimic their natural range of environmental parameters is crucial for the thriving culture of these organisms, the aquaculture industry frequently utilizes this as a live feed. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=87 SRC="FIGDIR/small/580272v1_ufig1.gif" ALT="Figure 1"> View larger version (36K): org.highwire.dtl.DTLVardef@ecae13org.highwire.dtl.DTLVardef@987662org.highwire.dtl.DTLVardef@1844230org.highwire.dtl.DTLVardef@158e4d_HPS_FORMAT_FIGEXP M_FIG C_FIG

Explore related subjects

ecologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

PILIYAN, R. P., Perumal, S., Barathan, B. P., Meril, D., Ragothaman, P., Shanmugam, G., Pachippan, P.. 2024-02-18. Molecular characterization and culture optimization of marine copepod Oithona dissimilis (Landberg, 1940) from Nagore coastal waters, Southern India. https://doi.org/10.1101/2024.02.14.580272

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Operationalising the context in regenerative agriculture: decision-making and farm variability

Soil degradation is a widespread challenge that requires a broad response at the individual farm level. To ensure effectivity, the practices should be tailored to the farm context: land manager objectives and farm specific challenges. These have however been difficult to quantify. Here we demonstrate that a workable farm context can be created based on a value survey, open satellite and soil data, and published models for vegetation gross primary productivity and soil erosion. Based on the findings, despite individual differences, farmers value profitability and operational efficiency, but also biodiversity and soil health. At least the regenerative farmers surveyed also value working for the greater good more than maintaining tradition or power. In spite of wide differences in farm production orientation, we also found that each farm also had a broad variation in individual fields GPP. Most fields have a stable GPP level, which is either high or low, and that there is a 2-3-fold difference between the weakest and best producing fields indicating the potential for improving GPP by improving the growing conditions on currently weak fields. In addition, soil loss was found to be highly concentrated in critical source areas, where 10% of the field area contributed to 50% of the soil loss. Overall, open data can be linked to modelling workflows to rapidly produce a decision-making context for farmers. This facilitates benchmarking and co-learning as well as enables land managers and advisors to identify the farm context for planning effective responses to soil degradation.

ecology↗
bioRxiv2026

Fly, land, listen: Autonomous intermittent locomotion enables scalable low-noise drone ecoacoustic surveys

Ecoacoustic monitoring is enabling scientists and land managers to monitor and manage biodiversity more effectively and cost-efficiently in the face of human pressures and rapidly changing climates. Currently, most ecoacoustic surveys use manually deployed static sensors to record data, limiting the scale and reach of surveying efforts. Here we present a proof-of-concept autonomous drone platform that can use intermittent locomotion to conduct ecoacoustic surveys using an onboard sensor. Our custom prototype is able to fly, navigate, and avoid obstacles autonomously, land at a pre-determined location, record audio from an onboard microphone whilst static, before taking off and moving to the next sampling site. Autonomous navigation and operation enable greater sampling flexibility, reach, and scalability. Furthermore, by recording audio only whilst landed, noise from the drone's rotors does not mask signals or disturb animals, simplifying signal processing and downstream ecological analyses. We conducted trials in a scrubland habitat at the Knepp Estate in West Sussex, where our prototype demonstrated successful autonomous navigation and obstacle avoidance. Furthermore, we found that avian biodiversity data collected from the drone platform was comparable to that from traditional static acoustic sensor deployments, and that vocalisation patterns were not significantly impacted by the noise of the drone arriving or leaving a site. While scaled deployments of our technology would require further technical and regulatory challenges to be solved, our first demonstration of autonomous intermittent robotics-assisted ecoacoustic surveys lays the foundations for more cost-effective and far-reaching biodiversity surveys, with transformative potential for conservation, agricultural management, biosecurity, and more.

ecology↗
bioRxiv2026

Do higher-order moments improve inference of population dynamics?

Fitting mathematical models of population dynamics to microbial time-series data allows us to estimate the ecological processes and interactions taking place in the microbiome. Repeated experiments of microbial systems yield replicates which slightly differ from each other. Some of this variability arises due to the fact that births and deaths occur at random. Most prior work focuses on fitting a deterministic mathematical model to the average across replicates. We use a stochastic model to fit the variability to the observed variability across replicates. Using a simulation-driven approach, we study the conditions under which our approach allows us to infer a larger fraction of ecological parameters correctly. We observe a substantial improvement in parameter inference. Lastly, our Bayesian approach not only allows us to incorporate prior information about the system, but also provides a distribution of parameters which conveys some idea of the uncertainty of the estimates.

ecology↗