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bioRxiv · 10.1101/2024.02.13.580159

THE POSSIBILITY OF THE USE OF LEUKOCYTES IN WEAK BLOOD GROUP ANTIGENS DETECTION

Kravchun, P.·Leontyeva, F.·Povelichenko, O.·Dielievska, V.
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Abstract

IntroductionMixed agglutination reaction is used for revealing A,B and H blood group antigens on the cells. The aim of the study was to visualize the antibody binding on weak blood group antigens of leukocytes in order to improve the quality of blood typing. Material and methodsThe antibodies were contacted with group A and B leukocytes at 4 {degrees}C. Mixed agglutination reaction was used to reveal group antigens on leukocytes using test erythrocytes. ResultsThe heated anti-A,B serum after adsorption on group A leukocytes agglutinated group A erythrocytes. Anti-A,B heated plasma after the contact with group B leukocytes agglutinated group B erythrocytes. Anti-A,B serum after adsorption on group B(A+) leukocytes agglutinated group B (A+) erythrocytes. Anti-A,B serum after adsorption on group A(B+) leukocytes agglutinated group B erythrocytes. ConclusionsMAR allowed to reveal both agglutinogenic and weak adsorbing blood group antigens, that helps to perform accurate blood group typing, especially for transplantation goals. The study revealed, the detection of adsorbing antigens of leukocytes should be performed at 4 {degrees}C.

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Kravchun, P., Leontyeva, F., Povelichenko, O., Dielievska, V.. 2024-02-17. THE POSSIBILITY OF THE USE OF LEUKOCYTES IN WEAK BLOOD GROUP ANTIGENS DETECTION. https://doi.org/10.1101/2024.02.13.580159

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