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bioRxiv · 10.1101/2024.02.07.579132

Degradation of PET Plastics by Wastewater Bacteria Engineered via Conjugation

Yip, A.·McArthur, O. D.·Ho, K. C.·Aucoin, M. G.·Ingalls, B. P.
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Abstract

Microplastics are contaminants of global concern that pose risks to ecosystems and human health. Focusing on PET plastics, we present a proof-of-concept for reduction of microplastic pollution: in situ engineering of bacteria in wastewater to degrade PET. Using a broad-host-range conjugative plasmid, we enabled various bacterial species from a municipal wastewater sample to express FAST-PETase, which was released into the extracellular environment. We found that FAST-PETase purified from some isolates could degrade about 40% of a 0.25 mm thick PET film within four days at 50 {degrees}C. We then demonstrate partial degradation of post-consumer PET over 5-7 days by exposure to conditioned media from isolates. These results have broad implications for addressing the global plastic pollution problem by enabling environmental bacteria to degrade PET plastics in situ.

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Yip, A., McArthur, O. D., Ho, K. C., Aucoin, M. G., Ingalls, B. P.. 2024-02-08. Degradation of PET Plastics by Wastewater Bacteria Engineered via Conjugation. https://doi.org/10.1101/2024.02.07.579132

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