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bioRxiv · 10.1101/2024.02.07.576204

Systematic Analysis of Immune Changes after B-cell Depletion in Autoimmune Multiple Sclerosis

Abstract

Multiple sclerosis (MS) is a complex genetically mediated autoimmune disease of the central nervous system where anti-CD20-mediated B cell depletion is remarkably effective in the treatment of early disease. While previous studies investigated the effect of B cell depletion on select immune cell subsets using flow cytometry-based methods, the therapeutic impact on patient immune landscape is unknown. In this study, we explored how a therapy-driven "in vivo perturbation" modulates the diverse immune landscape by measuring transcriptomic granularity with single-cell RNA sequencing (scRNAseq). We demonstrate that B cell depletion leads to cell type-specific changes in the abundance and function of CSF macrophages and peripheral blood monocytes. Specifically, a CSF-specific macrophage population with an anti-inflammatory transcriptomic signature and peripheral CD16+ monocytes increased in frequency post-B cell depletion. This was accompanied by increases in TNF messenger RNA and protein in monocytes post-B cell depletion, consistent with the finding that anti-TNF treatment exacerbates autoimmune activity in MS. In parallel, B cell depletion induced changes in peripheral CD4+ T cell populations, including increases in the frequency of TIGIT+ regulatory T cells and marked decreases in the frequency of myelin peptide loaded-tetramer binding CD4+ T cells. Collectively, this study provides an exhaustive transcriptomic map of immunological changes, revealing different mechanisms of action contributing to the high efficacy in B cell depletion treatment of MS.

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BibTeXRIS

Wei, J., Moon, J., Yasumizu, Y., Zhang, L., Radassi, K., Buitrago-Pocasangre, N., Deerhake, M. E., Strauli, N., Chan, A., Herman, A., Pedotte, R., Raposo, C., Tackenberg, B., Yim, I., Pappalardo, J., Longbrake, E. E., Sumida, T. S., Axisa, P.-P., Hafler, D. A.. 2024-02-09. Systematic Analysis of Immune Changes after B-cell Depletion in Autoimmune Multiple Sclerosis. https://doi.org/10.1101/2024.02.07.576204

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