scMicrobe PTA: Near Complete Genomes From Single Bacterial Cells

Bowers, R. M.; Gonzalez-Pena, V.; Wardhani, K.; Goudeau, D.; Blow, M. J.; Udwary, D.; Klein, D.; Vill, A. C.; Brito, I. L.; Woyke, T.; Malmstrom, R.; Gawad, C.

doi:10.1101/2024.01.30.577819

bioRxiv · 10.1101/2024.01.30.577819

scMicrobe PTA: Near Complete Genomes From Single Bacterial Cells

Bowers, R. M.·Gonzalez-Pena, V.·Wardhani, K.·Goudeau, D.·Blow, M. J.·Udwary, D.·Klein, D.·Vill, A. C.·Brito, I. L.·Woyke, T.·Malmstrom, R.·Gawad, C.

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Abstract

Microbial genomes produced by single-cell amplification are largely incomplete. Here, we show that primary template amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard amplification approaches were usually <30% complete. PTA-derived genomes also included more associated viruses and biosynthetic gene clusters.

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Bowers, R. M., Gonzalez-Pena, V., Wardhani, K., Goudeau, D., Blow, M. J., Udwary, D., Klein, D., Vill, A. C., Brito, I. L., Woyke, T., Malmstrom, R., Gawad, C.. 2024-01-31. scMicrobe PTA: Near Complete Genomes From Single Bacterial Cells. https://doi.org/10.1101/2024.01.30.577819

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