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bioRxiv · 10.1101/2024.01.29.577709

Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection

Hao, Q.·Shen, J.·Sang, L.·Bai, Y.·sun, j.
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Abstract

Tumor metastasis, responsible for approximately 90% of cancer-associated mortality, remains poorly understood. Here in this study we employed a melanoma lung metastasis model to screen for metastasis-related genes. By sequential tail vein injection of mouse melanoma B16F10 cells and the subsequently derived cells from lung metastasis into BALB/c mice, we successfully obtained highly metastatic B16F15 cells after five rounds of in vivo screening. RNA-sequencing analysis of B16F15 and B16F10 cells revealed a number of differentially expressed genes, some of these genes have previously been associated with tumor metastasis while others are novel discoveries. The identification of these metastasis-related genes not only improve our understanding of the metastasis mechanisms, but also provide potential diagnostic biomarkers and therapeutic targets for metastatic melanoma.

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Hao, Q., Shen, J., Sang, L., Bai, Y., sun, j.. 2024-01-31. Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection. https://doi.org/10.1101/2024.01.29.577709

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