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bioRxiv · 10.1101/2024.01.25.577189

Generation of human iPSC-derived phrenic-like motor neurons to model respiratory motor neuron degeneration in ALS

Thiry, L.·Sirois, J.·Durcan, T. M.·Stifani, S.
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Abstract

The fatal motor neuron (MN) disease Amyotrophic Lateral Sclerosis (ALS) is characterized by progressive MN degeneration. Phrenic MNs (phMNs) controlling the activity of the diaphragm are prone to degeneration in ALS, leading to death by respiratory failure. Understanding of the mechanisms of phMN degeneration in ALS is limited, mainly because human experimental models to study phMNs are lacking. Here we describe a method enabling the derivation of phrenic-like MNs from human iPSCs (hiPSC-phMNs) within 30 days. This protocol uses an optimized combination of small molecules followed by cell-sorting based on a cell-surface protein enriched in hiPSC-phMNs, and is highly reproducible using several hiPSC lines. We show further that hiPSC-phMNs harbouring ALS-associated amplification of the C9orf72 gene progressively lose their activity and undergo increased death compared to isogenic controls. These studies establish a previously unavailable protocol to generate human phMNs, offering a disease-relevant system to study mechanisms of respiratory MN dysfunction.

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Thiry, L., Sirois, J., Durcan, T. M., Stifani, S.. 2024-01-26. Generation of human iPSC-derived phrenic-like motor neurons to model respiratory motor neuron degeneration in ALS. https://doi.org/10.1101/2024.01.25.577189

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