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bioRxiv · 10.1101/2024.01.24.577149

High-speed two-photon microscopy with adaptive line-excitation

Li, Y.·Guo, S.·Mattison, B.·Yang, W.
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Abstract

We present a two-photon fluorescence microscope designed for high-speed imaging of neural activity in cellular resolution. Our microscope uses a new adaptive sampling scheme with line illumination. Instead of building images pixel by pixel via scanning a diffraction-limited spot across the sample, our scheme only illuminates the regions of interest (i.e., neuronal cell bodies), and samples a large area of them in a single measurement. Such a scheme significantly increases the imaging speed and reduces the overall laser power on the brain tissue. Using this approach, we performed high-speed imaging of the neural activity of mouse cortex in vivo. Our method provides a new sampling strategy in laser-scanning two-photon microscopy, and will be powerful for high-throughput imaging of neural activity.

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Li, Y., Guo, S., Mattison, B., Yang, W.. 2024-01-25. High-speed two-photon microscopy with adaptive line-excitation. https://doi.org/10.1101/2024.01.24.577149

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