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bioRxiv · 10.1101/2024.01.23.576811

Shear flow as a tool to distinguish microscopic activities of molecular machines in a chromatin loop

Kumar, S.·Padinhateeri, R.·Thakur, S.
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Abstract

Several types of molecular machines move along biopolymers like chromatin. However, the details about the microscopic activity of these machines and how to distinguish their modes of action are not well understood. We propose that the activity of such machines can be classified by studying looped chromatin under shear flow. Our simulations show that a chromatin-like polymer with two types of activities (constant or local curvature-dependent tangential forces) exhibits very different behavior under shear flow. We show that one can distinguish both activities by measuring the nature of a globule-to-extended coil transition, tank treading, and tumbling dynamics.

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Kumar, S., Padinhateeri, R., Thakur, S.. 2024-01-25. Shear flow as a tool to distinguish microscopic activities of molecular machines in a chromatin loop. https://doi.org/10.1101/2024.01.23.576811

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