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bioRxiv · 10.1101/2024.01.23.576810

Peptidoform analysis of IP-MS data allows detection of differentially present bait proteoforms

Sdelci, S.·Kourtis, S.·Cianferoni, D.·Serrano, L.
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Abstract

While it is recognised that protein functions are determined by their proteoform state, such as mutations and post-translational modifications, methods to determine their differential abundance between conditions are limited. Here, we present a novel workflow for classical immunoprecipitation coupled to mass spectrometry (IP-MS) data that focuses on identifying differential peptidoforms of the bait protein between conditions, providing additional information about protein function.

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Sdelci, S., Kourtis, S., Cianferoni, D., Serrano, L.. 2024-01-26. Peptidoform analysis of IP-MS data allows detection of differentially present bait proteoforms. https://doi.org/10.1101/2024.01.23.576810

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