Cell surface morphology mimicking nano-bio platform for immune cell stimulation

Varghese, B.; Gonzalez-Navarro, J. A.; Guerra, V. L. P.; Faizulina, M.; Artemieva, D.; Chum, T.; Ramakrishna, T. R. B.; Cebecauer, M.; Kovaricek, P.

doi:10.1101/2024.01.23.576453

bioRxiv · 10.1101/2024.01.23.576453

Cell surface morphology mimicking nano-bio platform for immune cell stimulation

Varghese, B.·Gonzalez-Navarro, J. A.·Guerra, V. L. P.·Faizulina, M.·Artemieva, D.·Chum, T.·Ramakrishna, T. R. B.·Cebecauer, M.·Kovaricek, P.

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Abstract

Studying the complex realm of cellular communication and interactions by fluorescence microscopy requires sample fixation on a transparent substrate. To activate T cells, which are pivotal in controlling the immune system, it is important to present the activating antigen in a spatial arrangement similar to the nature of the antigen-presenting cell, including the presence of ligands on microvilli. Similar arrangement is predicted for some other immune cells. In this work, immune cell-stimulating platform based on nanoparticle-ligand conjugates have been developed using a scalable, affordable, and broadly applicable technology, which can be readily deployed without the need for state-of-the-art nanofabrication instruments. The validation of surface biofunctionalization was performed by combination of fluorescence and atomic force microscopy techniques. We demonstrate that the targeted system serves as a biomimetic scaffold on which immune cells make primary contact with the microvilli-mimicking substrate and exhibit stimulus-specific activation.

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Varghese, B., Gonzalez-Navarro, J. A., Guerra, V. L. P., Faizulina, M., Artemieva, D., Chum, T., Ramakrishna, T. R. B., Cebecauer, M., Kovaricek, P.. 2024-01-24. Cell surface morphology mimicking nano-bio platform for immune cell stimulation. https://doi.org/10.1101/2024.01.23.576453

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