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bioRxiv · 10.1101/2024.01.21.576580

Exploring the potential of water channels for developing MRI reporters and sensors without the need for exogenous contrast agents

Abstract

Genetically encoded reporters for magnetic resonance imaging (MRI) offer a valuable technology for making molecular-scale measurements of biological processes within living organisms with high anatomical resolution and whole-organ coverage without relying on ionizing radiation. However, most MRI reporters rely on contrast agents, typically paramagnetic metals and metal complexes, which often need to be supplemented exogenously to create optimal contrast. To eliminate the need for contrast agents, we previously introduced aquaporin-1, a mammalian water channel, as a new reporter gene for the fully autonomous detection of genetically labeled cells using diffusion-weighted MRI. In this study, we aimed to expand the toolbox of diffusion-based genetic reporters by modulating aquaporin membrane trafficking and harnessing the evolutionary diversity of water channels across species. We identified a number of new water channels that functioned as diffusion-weighted reporter genes. In addition, we show that loss-of-function variants of yeast and human aquaporins can be leveraged to design first-in-class diffusion-based sensors for detecting the activity of a model protease within living cells.

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BibTeXRIS

Chacko, A. N., Miller, A. D. C., Dhanabalan, K. M., Mukherjee, A.. 2024-01-23. Exploring the potential of water channels for developing MRI reporters and sensors without the need for exogenous contrast agents. https://doi.org/10.1101/2024.01.21.576580

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