Decoration of Burkholderia Hcp1 protein to virus-like particles as a vaccine delivery platform.

Khakhum, N.; Baruch-Torres, N.; Stockton, J. L.; Chapartegui-Gonzalez, I. G.; Badten, A. J.; Adam, A.; Wang, T.; Huerta-Saquero, A.; Yin, W.; Torres, A. G.

doi:10.1101/2024.01.17.576127

bioRxiv · 10.1101/2024.01.17.576127

Decoration of Burkholderia Hcp1 protein to virus-like particles as a vaccine delivery platform.

Khakhum, N.·Baruch-Torres, N.·Stockton, J. L.·Chapartegui-Gonzalez, I. G.·Badten, A. J.·Adam, A.·Wang, T.·Huerta-Saquero, A.·Yin, W.·Torres, A. G.

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Abstract

Virus-like particles (VLPs) are protein-based nanoparticles frequently used as carrier in conjugate vaccine platforms. VLPs have been used to display foreign antigens for vaccination and to deliver immunotherapeutic against diseases. Hemolysin-coregulated proteins 1 (Hcp1) is a protein component of the Burkholderia type 6 secretion system which participates in intracellular invasion and dissemination. This protein has been reported as a protective antigen and is used in multiple vaccine candidates with various platforms against melioidosis, a severe infectious disease caused by the intracellular pathogen B. pseudomallei. In this study, we used P22 VLPs as a surface platform for decoration with Hcp1 using chemical conjugation. C57BL/6 mice were intranasally immunized with three doses of either PBS, VLPs or conjugated Hcp1-VLPs. Immunization with Hcp1-VLPs formulation induced Hcp1-specific-IgG, IgG1, IgG2c and IgA antibody responses. Furthermore, the serum from Hcp1-VLPs immunized mice enhanced the bacterial uptake and opsonophagocytosis by macrophages in the presence of complement. This study demonstrated an alternative strategy to develop a VLPs-based vaccine platform against Burkholderia species.

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Khakhum, N., Baruch-Torres, N., Stockton, J. L., Chapartegui-Gonzalez, I. G., Badten, A. J., Adam, A., Wang, T., Huerta-Saquero, A., Yin, W., Torres, A. G.. 2024-01-18. Decoration of Burkholderia Hcp1 protein to virus-like particles as a vaccine delivery platform.. https://doi.org/10.1101/2024.01.17.576127

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