Nationwide surveillance identifies yellow fever and chikungunya viruses transmitted by various species of Aedes mosquitoes in Nigeria

Nwangwu, U.; Oguzie, J. U.; Nwachukwu, W. E.; Onwude, C. O.; Dogunro, F. A.; Diallo, M.; Ezihe, C. K.; Agashi, N. O.; Eloy, E. I.; Anokwu, S. O.; Anioke, C. C.; Ikechukwu, L. C.; Nwosu, C. M.; Nwaogo, O. N.; Ngwu, I. M.; Onyeanusi, R. N.; Okoronkwo, A. I.; Orizu, F. U.; Etiki, M. O.; Onuora, E. N.; Adeorike, S. T.; Okeke, P. C.; Chukwuekezie, O. C.; Ochu, J. C.; Ibrahim, S. S.; Ifedayo, A.; Ihekweazu, C.; Happi, C.

doi:10.1101/2024.01.15.575625

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bioRxiv · 10.1101/2024.01.15.575625

Nationwide surveillance identifies yellow fever and chikungunya viruses transmitted by various species of Aedes mosquitoes in Nigeria

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Abstract

BackgroundSince its reemergence in 2017, yellow fever (YF) has been active in Nigeria. The Nigeria Centre for Disease Control (NCDC) has coordinated responses to the outbreaks with the support of the World Health Organization (WHO). The National Arbovirus and Vectors Research Centre (NAVRC) handles the vector component of these responses. This study sought to identify the vectors driving YF transmission and any of the targeted arboviruses and their distribution across states. MethodsEggs, larvae and pupae as well as adult mosquitoes were collected in observational, analytical, and cross-sectional surveys conducted in sixteen YF outbreak states between 2017 and 2020. Adult mosquitoes (field-collected or reared from immature stages) were morphologically identified, and arboviruses were detected using RT-qPCR at the African Centre of Excellence for Genomics of Infectious Diseases (ACEGID). ResultsAedes mosquitoes were collected in eleven of the sixteen states surveyed and the mosquitoes in nine states were found infected with arboviruses. A total of seven Aedes species were collected from different parts of the country. Aedes aegypti was the most dominant (51%) species, whereas Aedes africanus was the least (0.2%). Yellow fever virus (YFV) was discovered in 33 ([~]26%) out of the 127 Aedes mosquito pools. In addition to YFV, the Chikungunya virus (CHIKV) was found in nine pools. Except for Ae. africanus, all the Aedes species tested positive for at least one arbovirus. YFV-positive pools were found in six (6) Aedes species while CHIKV-positive pools were only recorded in two Aedes species. Edo State had the most positive pools (16), while Nasarawa, Imo, and Anambra states had the least (1 positive pool). Breteau and house indices were higher than normal transmission thresholds in all but one state. ConclusionIn Nigeria, there is a substantial risk of arbovirus transmission by Aedes mosquitoes, with YFV posing the largest threat at the moment. This risk is heightened by the fact that YFV and CHIKV have been detected in vectors across outbreak locations. Hence, there is an urgent need to step up arbovirus surveillance and control activities in the country. Author summaryEpidemics of arboviral infections are fast becoming the norm across Africa, especially in Nigeria, where sporadic epidemics of Aedes-borne Yellow Fever (YF) have been on the rise since 2017. A nationwide surveillance was conducted to detect the arboviruses and the vectors transmitting them during large YF outbreaks across Nigeria. Aedes mosquitoes collected from 57 local government areas/outbreak locations, covering 16 out of the 36 states in Nigeria, were reared to adulthood, morphologically identified to species level and assayed using qRT-PCR to detect six epidemiologically important arboviruses (Dengue, Chikungunya (CHIKV), Zika, YF, West Nile and Onyong nyong). Seven Aedes species (including Ae. aegypti, Ae. albopictus, Ae. circumluteolus, Ae. vittatus, Ae. simpsoni complex, Ae. luteocephalus, and Ae. africanus), were caught, with Ae. aegypti and Ae. albopictus the most widespread and predominant. Only YF and CHKV were found in mosquito pools from 9 states, with only Ae. aegypti pools positive for CHKV, while YF virus was found predominantly in this species and Ae. albopictus. This study provides a baseline for widespread distribution of the major Aedes vectors, and their arboviral transmission profiles, which can enhance evidence-based control measures, and strengthen nationwide surveillance systems to forestall future outbreaks of public health magnitude.

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Nwangwu, U., Oguzie, J. U., Nwachukwu, W. E., Onwude, C. O., Dogunro, F. A., Diallo, M., Ezihe, C. K., Agashi, N. O., Eloy, E. I., Anokwu, S. O., Anioke, C. C., Ikechukwu, L. C., Nwosu, C. M., Nwaogo, O. N., Ngwu, I. M., Onyeanusi, R. N., Okoronkwo, A. I., Orizu, F. U., Etiki, M. O., Onuora, E. N., Adeorike, S. T., Okeke, P. C., Chukwuekezie, O. C., Ochu, J. C., Ibrahim, S. S., Ifedayo, A., Ihekweazu, C., Happi, C.. 2024-01-15. Nationwide surveillance identifies yellow fever and chikungunya viruses transmitted by various species of Aedes mosquitoes in Nigeria. https://doi.org/10.1101/2024.01.15.575625

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