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bioRxiv · 10.1101/2024.01.12.575095

Dbp1 is a low performance paralog of RNA helicase Ded1 that drives impaired translation and heat stress response

Abstract

Ded1 and Dbp1 are paralogous conserved RNA helicases that enable translation initiation in yeast. Ded1 has been heavily studied but the role of Dbp1 is poorly understood. We find that the expression of these two helicases is controlled in an inverse and condition-specific manner. In meiosis and other long-term starvation states, Dbp1 expression is upregulated and Ded1 is downregulated, whereas in mitotic cells, Dbp1 expression is extremely low. Inserting the DBP1 ORF in place of the DED1 ORF cannot replace the function of Ded1 in supporting translation, partly due to inefficient mitotic translation of the DBP1 mRNA, dependent on features of its ORF sequence but independent of codon optimality. Global measurements of translation rates and 5 leader translation, activity of mRNA-tethered helicases, ribosome association, and low temperature growth assays show that--even at matched protein levels--Ded1 is more effective than Dbp1 at activating translation, especially for mRNAs with structured 5 leaders. Ded1 supports halting of translation and cell growth in response to heat stress, but Dbp1 lacks this function, as well. These functional differences in the ability to efficiently mediate translation activation and braking can be ascribed to the divergent, disordered N- and C-terminal regions of these two helicases. Altogether, our data show that Dbp1 is a "low performance" version of Ded1 that cells employ in place of Ded1 under long-term conditions of nutrient deficiency.

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BibTeXRIS

Powers, E. N., Reynaud, K., Sousa, C., Kuwayama, N., Jovanovic, M., Ingolia, N., Brar, G. A.. 2024-01-14. Dbp1 is a low performance paralog of RNA helicase Ded1 that drives impaired translation and heat stress response. https://doi.org/10.1101/2024.01.12.575095

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