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bioRxiv · 10.1101/2024.01.08.574706

Activity of Salmonella SPI-1 inhibits the TLR4-dependent transcriptional but not translational response during macrophage infection

Wood, G.·Johnson, R.·Brember, M.·Lastovka, F.·Tourlomousis, P.·Bryant, C.·Chung, B. Y.-W.
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Abstract

Changes in gene expression during bacterial infection are the combined result of altered transcription and translation, with the latter comparatively understudied. Gram-negative bacteria rapidly trigger cytokine gene transcription in macrophages through the activation of pathogen associated molecular pattern receptors, for example detection of Salmonella lipopolysaccharide (LPS) from the bacterial cell envelope by Toll-like receptor 4 (TLR4). Here, through time-resolved parallel translatomic and transcriptomic profiling, we now show temporal TLR4-specific translational upregulation of cell signalling proteins in macrophages induced by Salmonella. While transcriptional upregulation of these genes is dampened through the activity of the Salmonella SPI-1 type three secretion system, a robust translational response remains. These data reveal an important host-pathogen translational regulatory network that modifies the innate immune response of macrophages to infection.

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Wood, G., Johnson, R., Brember, M., Lastovka, F., Tourlomousis, P., Bryant, C., Chung, B. Y.-W.. 2024-01-09. Activity of Salmonella SPI-1 inhibits the TLR4-dependent transcriptional but not translational response during macrophage infection. https://doi.org/10.1101/2024.01.08.574706

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