Small non-coding RNA content in plasma-derived extracellular vesicles distinguish ataxic SCA3 mutation carriers from pre-ataxic and control subjects

Matos Santana, M.; Silva, P.; Pinto, M. M.; Gaspar, L.; Nobre, R. J.; Duarte, S.; Marques, T.; Gama-Carvalho, M.; Januario, C.; Cunha, I.; Ribeiro, J.; Huebener-Schmid, J.; Infante, J.; Raposo, M.; Lima, M.; Garcia-Moreno, H.; Giunti, P.; van de Warrenburg, B.; Synofzik, M.; Faber, J.; Klockgether, T.; ESMI Study Group,; Pereira de Almeida, L.

doi:10.1101/2024.01.04.574044

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bioRxiv · 10.1101/2024.01.04.574044

Small non-coding RNA content in plasma-derived extracellular vesicles distinguish ataxic SCA3 mutation carriers from pre-ataxic and control subjects

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Abstract

Spinocerebellar ataxia type 3 (SCA3), a neurodegenerative disorder caused by a CAG expansion in the ATXN3 gene, is the most common spinocerebellar ataxia subtype worldwide. Currently, there is no therapy to stop or prevent disease progression. Promising therapeutic strategies are emerging, but their translation into clinical practice requires sensitive and reliable biomarkers. Blood circulating extracellular vesicles constitute a promising source of biomarkers with potential to track alterations of the central nervous system due to their ability to cross the blood brain barrier. Here, we perform sequencing analysis of small RNAs from plasma-derived extracellular vesicles from SCA3 mutation carriers (10 pre-ataxic and 10 ataxic) and 12 control subjects to identify potential RNA biomarker candidates for this disease. Data showed that plasma-derived extracellular vesicles from ataxic SCA3 mutation carriers are enriched in mitochondrial, nuclear, and nucleolar RNA biotypes compared to pre-ataxic and control subjects. Moreover, ataxic mutation carriers could be discriminated from control and pre-ataxic subjects based on the miRNAs or piRNAs content, but not tRNA. Furthermore, we identified a subset of differentially expressed miRNAs and piRNAs that clearly differentiate ataxic mutation carriers from pre-ataxic and control subjects. These findings open new avenues for further investigation on the role of these RNAs in the pathogenesis of SCA3 and their potential as biomarkers for this disease.

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Matos Santana, M., Silva, P., Pinto, M. M., Gaspar, L., Nobre, R. J., Duarte, S., Marques, T., Gama-Carvalho, M., Januario, C., Cunha, I., Ribeiro, J., Huebener-Schmid, J., Infante, J., Raposo, M., Lima, M., Garcia-Moreno, H., Giunti, P., van de Warrenburg, B., Synofzik, M., Faber, J., Klockgether, T., ESMI Study Group,, Pereira de Almeida, L.. 2024-01-05. Small non-coding RNA content in plasma-derived extracellular vesicles distinguish ataxic SCA3 mutation carriers from pre-ataxic and control subjects. https://doi.org/10.1101/2024.01.04.574044

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