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bioRxiv · 10.1101/2023.12.28.573526

Interplay between Two Paralogous Human Silencing Hub (HuSH) Complexes in Regulating LINE-1 Element Silencing

Abstract

The Human Silencing Hub (HuSH) complex is composed of TASOR, MPP8, and PPHLN1 subunits and serves as a conserved protein complex responsible for silencing transposable elements in vertebrate animals. Despite its importance, the regulatory mechanisms and recruitment dynamics governing this complex remain poorly understood. In this study, we have identified a second HuSH complex, termed HuSH2, centered around TASOR2, a paralog of the core TASOR protein in HuSH. Our findings indicate that every subunit in both HuSH and HuSH2 has an important role in achieving precise genomic localization to distinct, non-overlapping genomic loci. We utilized in silico protein structure prediction to simulate the interactions between MPP8 and both TASOR paralogs. Drawing on the insights gained from these predictions, we implemented amino acid substitutions that interfered with the binding of MPP8 to each HuSH complex. Leveraging these MPP8 transgenes and other constructs, we identified an important role played by the relative quantities of HuSH complexes in controlling the activity of LINE-1 elements. Furthermore, our results suggest that dynamic changes in TASOR and TASOR2 expression enable cells to finely tune the extent of HuSH-mediated silencing. Our study provides insights into the intricate interplay between HuSH complexes, illuminating their important role in the regulation of retrotransposon silencing. Key PointsO_LIThe identification of a previously unknown HuSH2 complex, with TASOR2 as its central component. C_LIO_LIHuSH and HuSH2 complexes exhibit unique genomic localization patterns within the human genome. C_LIO_LIDisruption of the delicate balance between the two HuSH complexes results in the desilencing of LINE-1. C_LIO_LITASOR and TASOR2 engage in a competitive interaction for the HuSH subunit MPP8. C_LIO_LIThe localization of MPP8 to either HuSH or HuSH2 sites is intricately regulated by its interaction with TASOR and TASOR2. C_LI

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BibTeXRIS

Jensvold, Z. D., Christenson, A. E., Flood, J. R., Lewis, P. W.. 2023-12-28. Interplay between Two Paralogous Human Silencing Hub (HuSH) Complexes in Regulating LINE-1 Element Silencing. https://doi.org/10.1101/2023.12.28.573526

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