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bioRxiv · 10.1101/2023.12.21.572900

The Treatment of diarrheal mice with Tenebrio Molitor meal

Liu, t. t.·wang, Q. l.·wang, z. l.·yan, j. x.·zhu, j. j.·shen, h.·wang, j. g.
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Abstract

Feeding Tenebrio Molitor meal has an important effect on promoting the growth, absorption, reproduction, and disease resistance of animals. In this study, 3x108cfu/ml Escherichia coli was used to establish a mouse diarrhea model. Different doses (8%, 5%, 2.5%) of tenebrio molitor meal were added to the basic diet, respectively. The feed intake, water intake, body weight, loose stool rate, diarrhea rate, intestinal flora number, immune organ index, intestinal enzyme, and serum enzyme activities of the diarrhea mice were detected. The results showed that compared with the model group, the feed intake, water intake, and body weight of mice with diarrhea were improved by adding tenebrio molitor meal, and the dosage was proportional to that of tenebrio molitor meal. The rate of loose stool and diarrhea decreased with the increase of tenebrio molitor meal. The total bacterial count and Escherichia coli count in the intestinal tract of mice with diarrhea were negatively correlated with the dosage of tenebrio molitor meal. The immune organ index of the diarrhea mice in the three tenebrio molitor meal supplementation groups was higher than that in the model group and was proportional to the dosage. The liver index of the 8% tenebrio molitor meal supplementation group was 11.79mg/g higher than that in the blank group. Compared with the blank group, diarrhea significantly decreased the activities of various enzymes in the intestinal tract and serum of mice (P<0.05). Compared with the model group, the activities of intestinal and serum protective enzymes (superoxide dismutase, peroxidase, catalase), detoxification enzymes (glutathione-S transferase, acetylcholinesterase, acid phosphatase) and digestive enzymes (serum amylase, serum lipase, lactate dehydrogenase) in diarrhea mice were increased by adding tenebrio molitor meal (P<0.05). The results showed that tenebrio molitor meal had a positive effect on the treatment of diarrhea in mice.

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Liu, t. t., wang, Q. l., wang, z. l., yan, j. x., zhu, j. j., shen, h., wang, j. g.. 2023-12-23. The Treatment of diarrheal mice with Tenebrio Molitor meal. https://doi.org/10.1101/2023.12.21.572900

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