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bioRxiv · 10.1101/2023.12.21.572880

Genotypic and Phenotypic Analyses of Two Distinct Sets of Pseudomonas aeruginosa Urinary Tract Isolates

Ebrahim, H.·Haldenby, S.·Moore, M. P.·Dashti, A.·Floyd, R.·Fothergill, J.
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Abstract

Urinary tract infections (UTIs) are associated with a high burden of morbidity, mortality, and cost. Pseudomonas aeruginosa employs a myriad of virulence factors, including biofilm formation and motility mechanisms, to cause infections including persistent UTIs. P. aeruginosa is highly resistant to antibiotics and the World Health Organization has identified it as a pathogen for which novel antimicrobials are urgently required. Genotypic and phenotypic characterization of P. aeruginosa from UTIs are underreported. In addition, the rise of antimicrobial resistance (AMR) is a cause for concern, particularly in many countries where surveillance is severely lacking. 22 P. aeruginosa UTI isolates were sourced from the United Kingdom (UK) and Kuwait. To establish the phenotypes of UK isolates, growth analysis, biofilm formation assays, motility assays, and antibiotic disc diffusion assays were performed. Whole genome sequencing, antimicrobial susceptibility assays, and in silico detection of AMR-associated genes were conducted on both sets of isolates. In terms of their phenotypic characteristics and genomic composition, the UTI isolates varied. Multiple resistance genes associated with resistance to various classes of antibiotics, such as aminoglycosides, fluoroquinolones, and {beta}-lactams, particularly in isolates from Kuwait. Extreme antibiotic resistance was detected in the isolates obtained from Kuwait, indicating that the country may be an antibiotic resistance hotspot. This study highlights that isolates from UTIs are diverse and can display extremely high resistance. Surveillance in countries such as Kuwait are currently limited and this study suggest the need for greater surveillance.

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Ebrahim, H., Haldenby, S., Moore, M. P., Dashti, A., Floyd, R., Fothergill, J.. 2023-12-22. Genotypic and Phenotypic Analyses of Two Distinct Sets of Pseudomonas aeruginosa Urinary Tract Isolates. https://doi.org/10.1101/2023.12.21.572880

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