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bioRxiv · 10.1101/2023.12.19.572336

Structure of an insect gustatory receptor

Frank, H. M.·Walujkar, S.·Walsh, R. M.·Laursen, W. J.·Theobald, D. L.·Garrity, P. A.·Gaudet, R.
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Abstract

Gustatory Receptors (GRs) are critical for insect chemosensation and are potential targets for controlling pests and disease vectors. However, GR structures have not been experimentally determined. We present structures of Bombyx mori Gr9 (BmGr9), a fructose-gated cation channel, in agonist-free and fructose-bound states. BmGr9 forms a tetramer similar to distantly related insect Olfactory Receptors (ORs). Upon fructose binding, BmGr9s ion channel gate opens through helix S7b movements. In contrast to ORs, BmGR9s ligand-binding pocket, shaped by a kinked helix S4 and a shorter extracellular S3-S4 loop, is larger and solvent accessible in both agonist-free and fructose-bound states. Also unlike ORs, fructose binding by BmGr9 involves helix S5 and a binding pocket lined with aromatic and polar residues. Structure-based sequence alignments reveal distinct patterns of ligand-binding pocket residue conservation in GR subfamilies associated with distinct ligand classes. These data provide insight into the molecular basis of GR ligand specificity and function.

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Frank, H. M., Walujkar, S., Walsh, R. M., Laursen, W. J., Theobald, D. L., Garrity, P. A., Gaudet, R.. 2023-12-19. Structure of an insect gustatory receptor. https://doi.org/10.1101/2023.12.19.572336

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