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bioRxiv · 10.1101/2023.12.18.572124

The genome of the rayed Mediterranean limpet Patella caerulea (Linnaeus, 1758)

Halstead-Nussloch, G.·Signorini, S. G.·Giulio, M.·Crocetta, F.·Munari, M.·Della Torre, C.·Weber, A. A.-T.
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Abstract

Patella caerulea (Linnaeus, 1758) is a molluscan limpet species of the class Gastropoda. Endemic to the Mediterranean Sea, it is considered to be a keystone species in tidal and subtidal habitats due to its primary role in structuring and regulating the ecological balance of these habitats. It is currently being used as a bioindicator to assess the environmental quality of coastal marine waters and as a model species to understand adaptation to ocean acidification. Here we provide a high-quality reference genome assembly and annotation for Patella caerulea. We used a single specimen collected in the field to generate [~]30 Gb of PacBio HiFi data. The final assembly is 749.8 Mb large and contains 62 contigs, including the mitochondrial genome (14,938 bp). With an N50 of 48.8 Mb and 98% of the assembly contained in the 18 largest contigs, this assembly is near chromosome-scale. BUSCO scores were high (Mollusca: 87.8% complete; Metazoa: 97.2% complete) and similar to metrics observed for other chromosome-level Patella genomes, highlighting a possible bias in the Mollusca database for Patellids. We generated transcriptomic Illumina data from a second individual collected at the same locality, and used it together with protein evidence to annotate the genome. 23,938 protein coding gene models were found. By comparing this annotation with other published Patella annotations, we found that the distribution and median values of exon and gene lengths was comparable to other Patella species despite different annotation approaches. The present high-quality Patella caerulea reference genome is an important resource for future ecological and evolutionary studies. SignificanceReference genomes are essential resources for biodiversity conservation and management. Patella caerulea (Linnaeus, 1758) is a gastropod species occurring in the Mediterranean Sea that is currently used as a model to understand the impact of pollution and ocean acidification on marine biodiversity. Here we present a high-quality reference genome of P. caerulea, that almost reaches chromosome-level contiguity. We further provide a high-quality genome annotation supported by transcriptomic evidence. This reference genome will be of interest for researchers working on the ecology and evolution of marine biodiversity. All data is available on the public database NCBI for future use by researchers.

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Halstead-Nussloch, G., Signorini, S. G., Giulio, M., Crocetta, F., Munari, M., Della Torre, C., Weber, A. A.-T.. 2023-12-18. The genome of the rayed Mediterranean limpet Patella caerulea (Linnaeus, 1758). https://doi.org/10.1101/2023.12.18.572124

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