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bioRxiv · 10.1101/2023.12.15.571964

Imaging the voltage of neurons distributed across entire brains of larval zebrafish

Wang, Z.·Zhang, J.·Symvoulidis, P.·Guo, W.·Zhang, L.·Wilson, M. A.·Boyden, E. S.
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Abstract

Neurons interact in networks distributed throughout the brain. Although much effort has focused on whole-brain calcium imaging, recent advances in genetically encoded voltage indicators (GEVIs) raise the possibility of imaging voltage of neurons distributed across brains. To achieve this, a microscope must image at high volumetric rate and signal-to-noise ratio. We present a remote scanning light-sheet microscope capable of imaging GEVI-expressing neurons distributed throughout entire brains of larval zebrafish at a volumetric rate of 200.8 Hz. We measured voltage of [~]1/3 of the neurons of the brain, distributed throughout. We observed that neurons firing at different times during a sequence were located at different brain locations, for sequences elicited by a visual stimulus, which mapped onto locations throughout the optic tectum, as well as during stimulus-independent bursts, which mapped onto locations in the cerebellum and medulla. Whole-brain voltage imaging may open up frontiers in the fundamental operation of neural systems.

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Wang, Z., Zhang, J., Symvoulidis, P., Guo, W., Zhang, L., Wilson, M. A., Boyden, E. S.. 2023-12-16. Imaging the voltage of neurons distributed across entire brains of larval zebrafish. https://doi.org/10.1101/2023.12.15.571964

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