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bioRxiv · 10.1101/2023.12.15.571646

Parallel Molecular Data Storage by Printing Epigenetic Bits on DNA

Zhang, C.·Wu, R.·Sun, F.·Lin, Y.·Zhang, Y.·Liang, Y.·Chen, Y.·Teng, J.·Song, Z.·Liu, N.·Ouyang, Q.·Qian, L.·Yan, H.
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Abstract

DNA storage has shown potential to transcend current silicon-based data storage technologies in storage density, lifetime and energy consumption. However, writing large-scale data directly into DNA sequences by de novo synthesis remains uneconomical in time and cost. Inspired by the natural genomic modifications, in this work, we report an alternative, parallel strategy that enables the writing of arbitrary data on DNA using premade nucleic acids. With epigenetic modifications as information bits, our system employed DNA self-assembly guided enzymatic methylation to perform movable-type printing on universal DNA templates. By programming with a finite set of 700 DNA movable types and 5 templates, we achieved the synthesis-free writing of [~]270000 bits on an automated platform with 350 bits written per reaction. The data encoded in complex epigenetic patterns were retrieved high-throughput by nanopore sequencing, and algorithms were developed to finely resolve 240 modification patterns per sequencing reaction. Our framework presents a new modality of DNA-based data storage that is parallel, programmable, stable and scalable. Such a modality opens up avenues towards practical data storage and dual-mode data functions in biomolecular systems.

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Zhang, C., Wu, R., Sun, F., Lin, Y., Zhang, Y., Liang, Y., Chen, Y., Teng, J., Song, Z., Liu, N., Ouyang, Q., Qian, L., Yan, H.. 2023-12-15. Parallel Molecular Data Storage by Printing Epigenetic Bits on DNA. https://doi.org/10.1101/2023.12.15.571646

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