Temporal Changes in Innate and Adaptive Immunity During Sepsis as Determined by ELISpot

Walton, A. H.; Hotchkiss, R. S.; Osborne, D.; Unsinger, J.; Han, E. H.; Sheets, L.; Mazer, M. B.; Remy, K. E.; Griffith, T. S.; Rao, M.; Badovinac, V. P.; Brakenridge, S. C.; Turnbull, I.; Efron, P. A.; Moldawer, L. L.; Calwell, C. C.

doi:10.1101/2023.12.14.571668

bioRxiv · 10.1101/2023.12.14.571668

Temporal Changes in Innate and Adaptive Immunity During Sepsis as Determined by ELISpot

Walton, A. H.·Hotchkiss, R. S.·Osborne, D.·Unsinger, J.·Han, E. H.·Sheets, L.·Mazer, M. B.·Remy, K. E.·Griffith, T. S.·Rao, M.·Badovinac, V. P.·Brakenridge, S. C.·Turnbull, I.·Efron, P. A.·Moldawer, L. L.·Calwell, C. C.

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Abstract

BackgroundThe inability to evaluate host immunity in a rapid quantitative manner in patients with sepsis has severely hampered development of novel immune therapies. The ELISpot assay is a functional bioassay that measures the number of cytokine-secreting cells and the relative amount of cytokine produced at the single-cell level. A key advantage of ELISpot is its excellent dynamic range enabling a more precise quantifiable assessment of host immunity. Herein, we tested the hypothesis on whether the ELISpot assay can detect dynamic changes in both innate and adaptive immunity as they often occur during sepsis. We also tested whether ELISpot could detect the effect of immune drug therapies to modulate innate and adaptive immunity. MethodsMice were made septic using sublethal cecal ligation and puncture (CLP). Blood and spleens were harvested serially and ex vivo IFN-{gamma} and TNF- production were compared by ELISpot and ELISA. The capability of ELISpot to detect changes in innate and adaptive immunity due to in vivo immune therapy with dexamethasone, IL-7, and arginine was also evaluated. ResultsELISpot confirmed a decreased innate and adaptive immunity responsiveness during sepsis progression. More importantly, ELISpot was also able to detect changes in adaptive and innate immunity in response to immune-modulatory reagents, for example dexamethasone, arginine, and IL-7 in a readily quantifiable manner, as predicted by the reagents known mechanisms of action. ELISpot and ELISA results tended to parallel one another although some differences were noted. ConclusionELISpot offers a unique capability to assess the functional status of both adaptive and innate immunity over time. The results presented herein demonstrate that ELISpot can also be used to detect and follow the in vivo effects of drugs to ameliorate sepsis-induced immune dysfunction. This capability would be a major advance in guiding new immune therapies in sepsis.

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Walton, A. H., Hotchkiss, R. S., Osborne, D., Unsinger, J., Han, E. H., Sheets, L., Mazer, M. B., Remy, K. E., Griffith, T. S., Rao, M., Badovinac, V. P., Brakenridge, S. C., Turnbull, I., Efron, P. A., Moldawer, L. L., Calwell, C. C.. 2023-12-14. Temporal Changes in Innate and Adaptive Immunity During Sepsis as Determined by ELISpot. https://doi.org/10.1101/2023.12.14.571668

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