snPATHO-seq: unlocking the pathology archives

Wang, T.; Harvey, K.; Escudero Morales, J.; Kiedik, B.; Al-Eryani, G.; Greenwald, A. C.; Kalavros, N.; SegatovDezem, F.; Ma, Y.; Pita-Juarez, Y.; Wise, K.; Degletagne, C.; Elz, A.; Johanneson, J.; Pakiam, F.; Ryu, H.; Newell, E. W.; Tonon, L.; Kohlway, A.; Drennon, T.; Hadadianpour, A.; Abousoud, J.; Scott, R.; Lund, P.; Durruthy, J.; Vallejos, A. F.; Kaczorowski, D.; Warren, J.; Butler, L. M.; O'Toole, S.; Plummer, J. T.; Vlachos, I.; Lundeberg, J.; Swarbrick, A.; Martelotto, L. G.

doi:10.1101/2023.12.07.570700

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bioRxiv · 10.1101/2023.12.07.570700

snPATHO-seq: unlocking the pathology archives

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Abstract

Formalin-fixed paraffin-embedded (FFPE) samples are valuable but underutilized in single-cell omics research due to their low DNA and RNA quality. In this study, leveraging recent single-cell genomic technology advances, we introduce a versatile method to derive high-quality single-nucleus transcriptomic data from FFPE samples.

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Wang, T., Harvey, K., Escudero Morales, J., Kiedik, B., Al-Eryani, G., Greenwald, A. C., Kalavros, N., SegatovDezem, F., Ma, Y., Pita-Juarez, Y., Wise, K., Degletagne, C., Elz, A., Johanneson, J., Pakiam, F., Ryu, H., Newell, E. W., Tonon, L., Kohlway, A., Drennon, T., Hadadianpour, A., Abousoud, J., Scott, R., Lund, P., Durruthy, J., Vallejos, A. F., Kaczorowski, D., Warren, J., Butler, L. M., O'Toole, S., Plummer, J. T., Vlachos, I., Lundeberg, J., Swarbrick, A., Martelotto, L. G.. 2023-12-08. snPATHO-seq: unlocking the pathology archives. https://doi.org/10.1101/2023.12.07.570700

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