Sequence reliance of a Drosophila context-dependent transcription factor

Hodkinson, L. J.; Gross, J.; Schmidt, C. A.; Diaz-Saldana, P. P.; Aoki, T.; Rieder, L. E.

doi:10.1101/2023.12.07.570650

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bioRxiv · 10.1101/2023.12.07.570650

Sequence reliance of a Drosophila context-dependent transcription factor

Hodkinson, L. J.·Gross, J.·Schmidt, C. A.·Diaz-Saldana, P. P.·Aoki, T.·Rieder, L. E.

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Abstract

Despite binding similar cis elements in multiple locations, a single transcription factor often performs context-dependent functions at different loci. How factors integrate cis sequence and genomic context is still poorly understood and has implications for off-target effects in genetic engineering. The Drosophila context-dependent transcription factor CLAMP targets similar GA-rich cis elements on the X-chromosome and at the histone gene locus but recruits very different, loci-specific factors. We discover that CLAMP leverages information from both cis element and local sequence to perform context-specific functions. Our observations imply the importance of other cues, including protein-protein interactions and the presence of additional cofactors.

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Hodkinson, L. J., Gross, J., Schmidt, C. A., Diaz-Saldana, P. P., Aoki, T., Rieder, L. E.. 2023-12-11. Sequence reliance of a Drosophila context-dependent transcription factor. https://doi.org/10.1101/2023.12.07.570650

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