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bioRxiv · 10.1101/2023.12.04.569849

Transcriptomic correlates of state modulation in forebrain interneurons: A cross-species analysis

Keijser, J.·Hertaeg, L.·Sprekeler, H.
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Abstract

GABAergic inhibitory interneurons comprise many subtypes that differ in their molecular, anatomical and functional properties. In mouse visual cortex, they also differ in their modulation with an animals behavioural state, and this state modulation can be predicted from the first principal component (PC) of the gene expression matrix. Here, we ask whether this link between transcriptome and state-dependent processing generalises across species. To this end, we analysed seven single-cell and single-nucleus RNA sequencing datasets from mouse, human, songbird, and turtle forebrains. Despite homology at the level of cell types, we found clear differences between transcriptomic PCs, with greater dissimilarities between evolutionarily distant species. These dissimilarities arise from two factors: divergence in gene expression within homologous cell types and divergence in cell type abundance. We also compare the expression of cholinergic receptors, which are thought to causally link transcriptome and state modulation. Several cholinergic receptors predictive of state modulation in mouse interneurons are differentially expressed between species. Circuit modelling and mathematical analyses suggest conditions under which these expression differences could translate into functional differences.

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Keijser, J., Hertaeg, L., Sprekeler, H.. 2023-12-04. Transcriptomic correlates of state modulation in forebrain interneurons: A cross-species analysis. https://doi.org/10.1101/2023.12.04.569849

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