Identification of potential riboswitch elements in Homo Sapiens mRNA 5'UTR sequences using Positive-Unlabeled Machine learning

Abstract

Riboswitches are a class of noncoding RNA structures that interact with target ligands to cause a conformational change that can then execute some regulatory purpose within the cell. Riboswitches are ubiquitous and well characterized in bacteria and prokaryotes, with additional examples also being found in fungi, plants, and yeast. To date, no purely RNA-small molecule riboswitch has been discovered in Homo Sapiens. Several analogous riboswitch-like mechanisms have been described within the H. Sapiens translatome within the past decade, prompting the question: Is there a H. Sapiens riboswitch dependent on only small molecule ligands? In this work, we set out to train positive unlabeled machine learning classifiers on known riboswitch sequences and apply the classifiers to H. Sapiens mRNA 5UTR sequences found in the 5UTR database, UTRdb, in the hope of identifying a set of mRNAs to investigate for riboswitch functionality. 67,683 riboswitch sequences were obtained from RNAcentral and sorted for ligand type and used as positive examples and 48,031 5UTR sequences were used as unlabeled, unknown examples. Positive examples were sorted by ligand, and 20 positive-unlabeled classifiers were trained on sequence and secondary structure features while withholding one or two ligand classes. Cross validation was then performed on the withheld ligand sets to obtain a validation accuracy range of 75%-99%. The joint sets of 5UTRs identified as potential riboswitches by the 20 classifiers were then analyzed. 15333 sequences were identified as a riboswitch by one or more classifier(s) and 436 of the H. Sapiens 5UTRs were labeled as harboring potential riboswitch elements by all 20 classifiers. These 436 sequences were mapped back to the most similar riboswitches within the positive data and examined. An online database of identified and ranked 5UTRs, their features, and their most similar matches to known riboswitches, is provided to guide future experimental efforts to identify H. Sapiens riboswitches. Author summaryRiboswitches are an important regulatory element mostly found in bacteria that have not been described in Homo Sapiens. However, if human riboswitches exist and if they can be found, they could have vast implications on human disease. We apply positive-unlabeled machine learning to on known riboswitch sequences to search H. Sapiens 5UTR sequences for potential riboswitches. We analyze our ensemble predictions for likely H. Sapiens 5UTR riboswitches using GO analysis to determine their potential functional roles, and we rank and display our predicted sequences next to the most similar known riboswitches. We expect these analyses to be helpful to the scientific community in planning future experiments for laboratory discovery and validation. 0.1 Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=169 SRC="FIGDIR/small/568398v2_ufig1.gif" ALT="Figure 1"> View larger version (55K): org.highwire.dtl.DTLVardef@87c27org.highwire.dtl.DTLVardef@10f085corg.highwire.dtl.DTLVardef@1edfcaborg.highwire.dtl.DTLVardef@1674ce0_HPS_FORMAT_FIGEXP M_FIG C_FIG