Impact of blood meals taken on ivermectin-treated livestock on survival and fecundity of the malaria vector Anopheles coluzzii under laboratory conditions

Pooda, S. H.; Hien, D. F. d. S.; Pagabeleguem, S.; Heinrich, A. P.; Porciani, A.; Sagna, A. B.; Zela, L.; Percoma, L.; Lefevre, T.; Dabire, R. K.; Koffi, A. A.; Düering, R.-A.; Pennetier, C.; Moiroux, N.; Mouline, K.

doi:10.1101/2023.11.22.568228

bioRxiv · 10.1101/2023.11.22.568228

Impact of blood meals taken on ivermectin-treated livestock on survival and fecundity of the malaria vector Anopheles coluzzii under laboratory conditions

Pooda, S. H.·Hien, D. F. d. S.·Pagabeleguem, S.·Heinrich, A. P.·Porciani, A.·Sagna, A. B.·Zela, L.·Percoma, L.·Lefevre, T.·Dabire, R. K.·Koffi, A. A.·Düering, R.-A.·Pennetier, C.·Moiroux, N.·Mouline, K.

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Abstract

Treatment of livestock with endectocides such as ivermectin is viewed as a complementary vector control approach to address residual transmission of malaria. However, efficacy of this treatment may vary between animal species. Hence, our purpose was to investigate the effects of ivermectin treatments of common livestock species on life history traits of the opportunistic malaria vector Anopheles coluzzii. Sheep, goats and pigs were treated using injectable veterinary ivermectin formulation at the species-specific doses (recommended dose for all species and high dose in pig). Mosquito batches were exposed to treated and control (not injected) animals at different days after treatment. Daily mosquito mortality was recorded and fecundity assessed through the count of gravid females and the number of eggs they developed. The recommended dose of ivermectin induced a significant decrease in mosquito survival for up to 7 days after injection (DAI), with a decrease of 89.7%, 66.7%, and 48.4% in treated pigs, goats and sheep, respectively, compared to control animals. In treated pigs, the triple therapeutic dose decreased mosquito survival of 68.97% relatively to controls up to 14 DAI. The average number in gravid females Anopheles that survived after feeding on treated animals were reduced when blood-meals were taken on sheep (2.57% and 42.03% at 2 and 7 DAI), or on goats (decrease of the 28.28% and 73.64% respectively at 2 and 7 DAI). This study shows that ivermectin treatments to animals negatively impacts An. coluzzii life history traits and could reduce vector densities in areas where livestock live near humans. However, due to short-term efficacy of single dose treatments, repeated treatments and potentially increased dosages would be required to span the transmission season. The use of long-acting ivermectin formulations is discussed as a mean for extending efficacy while remaining cost effective.

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Pooda, S. H., Hien, D. F. d. S., Pagabeleguem, S., Heinrich, A. P., Porciani, A., Sagna, A. B., Zela, L., Percoma, L., Lefevre, T., Dabire, R. K., Koffi, A. A., Düering, R.-A., Pennetier, C., Moiroux, N., Mouline, K.. 2023-11-22. Impact of blood meals taken on ivermectin-treated livestock on survival and fecundity of the malaria vector Anopheles coluzzii under laboratory conditions. https://doi.org/10.1101/2023.11.22.568228

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