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bioRxiv · 10.1101/2023.11.21.568001

Exploring functional conservation in silico: a new machine learning approach to RNA-editing

Abstract

Around 50 years from now, molecular biology opened the path to understand changes in forms, adaptations, complexity, or the basis of human diseases, through myriads of reports on gene birth, gene duplication, gene expression regulation, and splicing regulation, among other relevant mechanisms behind gene function. Here, with the advent of big data and artificial intelligence (AI), we focus on an elusive and intriguing mechanism of gene function regulation, RNA editing, in which a single nucleotide from an RNA molecule is changed with a remarkable impact in the increase of the complexity of transcriptome and proteome. We present a new generation approach to assess the functional conservation of the RNA-editing targeting mechanism using two AI learning algorithms, random forest (RF) and bidirectional long short-term memory (biLSTM) neural networks with attention layer. These algorithms combined with RNA-editing data coming from databases and variant calling from same-individual RNA and DNA-seq experiments from different species, allowed us to predict RNA-editing events using both primary sequence and secondary structure. Then, we devised a method for assessing conservation or divergence in the molecular mechanisms of editing completely in silico: the cross-training analysis. This novel method not only helps to understand the conservation of the editing mechanism through evolution but could set the basis for understanding how it is involved in several human diseases.

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BibTeXRIS

Zawisza-Alvarez, M., Penuela-Melero, J., Vegas, E., Reverter, F., Garcia-Fernandez, J., Herrera-Ubeda, C.. 2023-11-22. Exploring functional conservation in silico: a new machine learning approach to RNA-editing. https://doi.org/10.1101/2023.11.21.568001

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