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bioRxiv · 10.1101/2023.11.21.565821

Hi-C2B: Optimised detection of chromosomal contacts within synchronised meiotic S. cerevisiae cells

Wright, E. M.·Schalbetter, S. A.·Neale, M. J.
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Abstract

Hi-C, a genome-wide chromosome conformation capture assay is a powerful tool used to study three-dimensional genome organisation by converting physical pairwise interactions into counts of pairwise interaction. To study the many temporally regulated facets of meiotic recombination in S. cerevisiae the Hi-C assay must be robust such that fine- and wide-scale comparisons between genetic datasets can be made. Here we describe an updated protocol for Hi-C (Hi-C2B) that generates reproducible libraries of interaction data with low noise and for a relatively low cost.

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Wright, E. M., Schalbetter, S. A., Neale, M. J.. 2023-11-22. Hi-C2B: Optimised detection of chromosomal contacts within synchronised meiotic S. cerevisiae cells. https://doi.org/10.1101/2023.11.21.565821

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