An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses

Andersson, L.; Rubin, C.-J.; Hodge, M.; Naboulsi, R.; Beckman, M.; Bellone, R. R.; Kallenberg, A.; Usrey, S. J.; Ohmura, H.; Seki, K.; Ohnuma, A.; Davis, B. W.; Tozaki, T.; Lindgren, G.

doi:10.1101/2023.11.05.565619

bioRxiv · 10.1101/2023.11.05.565619

An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses

Andersson, L.·Rubin, C.-J.·Hodge, M.·Naboulsi, R.·Beckman, M.·Bellone, R. R.·Kallenberg, A.·Usrey, S. J.·Ohmura, H.·Seki, K.·Ohnuma, A.·Davis, B. W.·Tozaki, T.·Lindgren, G.

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Abstract

The Greying with age phenotype involves loss of hair pigmentation whereas skin pigmentation is not reduced and a predisposition to melanoma. The causal mutation was initially reported as a duplication of a 4.6 kb intronic sequence in Syntaxin 17. The speed of greying varies considerably among Grey horses. Here we demonstrate the presence of two different Grey alleles, G2 carrying two tandem copies of the duplicated sequence and G3 carrying three. The latter is by far the most common allele, probably due to strong selection for the striking white phenotype. Our results reveal a remarkable dosage effect where the G3 allele is associated with fast greying and high incidence of melanoma whereas G2 is associated with slow greying and low incidence of melanoma. Epigenetic analysis, based on nanopore sequencing of genomic DNA, reveals a drastic reduction in DNA methylation in part of the duplicated sequence harboring MITF binding sites. The copy number expansion transforms a weak enhancer to a strong melanocyte-specific enhancer that underlies hair greying (G2 and G3) and a drastically elevated risk of melanoma (G3 only).

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Andersson, L., Rubin, C.-J., Hodge, M., Naboulsi, R., Beckman, M., Bellone, R. R., Kallenberg, A., Usrey, S. J., Ohmura, H., Seki, K., Ohnuma, A., Davis, B. W., Tozaki, T., Lindgren, G.. 2023-11-05. An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses. https://doi.org/10.1101/2023.11.05.565619

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