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bioRxiv · 10.1101/2023.11.03.565572

Loss of ninein interferes with osteoclast formation and causes premature ossification

Abstract

Ninein is a centrosome protein that has been implicated in microtubule anchorage and centrosome cohesion. Mutations in the human ninein gene have been linked to Seckel syndrome and to a rare form of skeletal dysplasia. However, the role of ninein in skeletal development remains unknown. Here, we describe a ninein knockout mouse with advanced endochondral ossification during embryonic development. Although the long bones maintain a regular size, the absence of ninein delays the formation of the bone marrow cavity in the prenatal tibia. Likewise, intramembranous ossification in the skull is more developed, leading to a premature closure of the interfrontal suture. We demonstrate that ninein is strongly expressed in osteoclasts of control mice, and that its absence reduces the fusion of precursor cells into syncytial osteoclasts. As a consequence, ninein-deficient osteoclasts have a reduced capacity to resorb bone. At the cellular level, the absence of ninein interferes with centrosomal microtubule organization, reduces centrosome cohesion, and provokes the loss of centrosome clustering in multinucleated mature osteoclasts. We propose that centrosomal ninein is important for osteoclast fusion, to enable a functional balance between bone-forming osteoblasts and bone-resorbing osteoclasts during skeletal development.

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BibTeXRIS

Gilbert, T., Barbier, M., Duployer, B., Dufrancais, O., Martet, L.-E., Dalbard, E., Segot, L., Tenailleau, C., Verollet, C., Bierkamp, C., Merdes, A.. 2023-11-06. Loss of ninein interferes with osteoclast formation and causes premature ossification. https://doi.org/10.1101/2023.11.03.565572

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