CC-seq: Nucleotide-resolution mapping of Spo11 DNA double-strand breaks in S. cerevisiae cells

Brown, G. G. B.; Gittens, W. H.; Allison, R. M.; Oliver, A. W.; Neale, M. J.

doi:10.1101/2023.10.31.564935

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bioRxiv · 10.1101/2023.10.31.564935

CC-seq: Nucleotide-resolution mapping of Spo11 DNA double-strand breaks in S. cerevisiae cells

Brown, G. G. B.·Gittens, W. H.·Allison, R. M.·Oliver, A. W.·Neale, M. J.

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Abstract

i.During meiosis Spo11 generates DNA double-strand breaks to induce recombination, becoming covalently attached to the 5' ends on both sides of the break during this process. Such Spo11 "covalent complexes" are transient in wild-type cells, but accumulate in nuclease mutants unable to initiate repair. The CC-seq method presented here details how to map the location of these Spo11 complexes genome-wide with strand-specific nucleotide-resolution accuracy in synchronised Saccharomyces cerevisiae meiotic cells.

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Brown, G. G. B., Gittens, W. H., Allison, R. M., Oliver, A. W., Neale, M. J.. 2023-11-02. CC-seq: Nucleotide-resolution mapping of Spo11 DNA double-strand breaks in S. cerevisiae cells. https://doi.org/10.1101/2023.10.31.564935

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